Publication Detail
| Citation : |
Collier AC, Thevenon AD, Goh W, Hiraoka M, Kendal-Wright CE. (2015)
Placental profiling of UGT1A enzyme expression and activity and interactions with preeclampsia at term.
Eur J Drug Metab Pharmacokinet 40(4):471-80.
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| Abstract : |
Placental UDP-glucuronosyltransferase (UGT) enzymes have critical roles in hormone, nutrient, chemical balance and fetal exposure during pregnancy. Placental UGT1A isoforms were profiled and differences between preeclamptic (PE) and non-PE placental UGT expression determined. In third trimester villous placenta, UGT1A1, 1A4, 1A6 and 1A9 were expressed and active in all specimens (n�?� =�?� 10), but UGT1A3, 1A5, 1A7, 1A8 and 1A10 were absent. The UGT1A activities were comparable to human liver microsomes per milligram, but placental microsome yields were only 2�?� % of liver (1�?� mg/g of tissue vs. 45�?� mg/g of tissue). For successful PCR, placental collection and processing within�?� 60�?� min from delivery, including DNAse and �¢?�¥300�?� ng of RNA in reverse transcription were essential and snap freezing in liquid nitrogen immediately was the best preservation method. Although UGT1A6 mRNA was lower in PE (P�?� <�?� 0.001), there were no other significant effects on UGT mRNA, protein or activities. A more comprehensive tissue sample set is required for confirmation of PE interactions with UGT. Placental UGT1A enzyme expression patterns are similar to the liver and a detoxicative role for placental UGT1A is inferred.
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| URL Link : |
http://www.ncbi.nlm.nih.gov/pubmed/25465229
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| PMID : |
25465229
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| PMCID : |
PMC4536191
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Supported by a grant from the National Institute on Minority Health and Health Disparities (U54MD007584), National Institutes of Health.
