Publication Detail

Citation : Horton JS, Wakano CT, Speck M, Stokes AJ. (2015)
Two-pore channel 1 interacts with citron kinase, regulating completion of cytokinesis.
Channels (Austin) 9(1):21-9.
Abstract : Two-pore channels (TPC1, 2, and 3) are recently identified endolysosmal ion channels, but remain poorly characterized. In this study, we show for the first time a role for TPC1 in cytokinesis, the final step in cell division. HEK 293 T-REx cells inducibly overexpressing TPC1 demonstrated a lack of proliferation accompanied by multinucleation and an increase in G2/M cycling cells. Increased TPC1 was associated with a concomitant accumulation of active RhoGTP and a decrease in phosphorylated myosin light chain (MLC). Finally, we demonstrated a novel interaction between TPC1 and citron kinase (CIT). These results identify TPC1 as a central component of cytokinetic control, specifically during abscission, and introduce a means by which the endolysosomal system may play an active role in this process.
URL Link :
PMID : 25665131
PMCID : PMC4594595
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