Publication Detail
| Citation : |
Greineisen WE, Speck M, Shimoda LM, Sung C, Phan N, Maaetoft-Udsen K, Stokes AJ, Turner H. (2014)
Lipid body accumulation alters calcium signaling dynamics in immune cells.
Cell Calcium 56(3):169-80.
|
| Abstract : |
There is well-established variability in the numbers of lipid bodies (LB) in macrophages, eosinophils, and neutrophils. Similarly to the steatosis observed in adipocytes and hepatocytes during hyperinsulinemia and nutrient overload, immune cell LB hyper-accumulate in response to bacterial and parasitic infection and inflammatory presentations. Recently we described that hyperinsulinemia, both in vitro and in vivo, drives steatosis and phenotypic changes in primary and transformed mast cells and basophils. LB reach high numbers in these steatotic cytosols, and here we propose that they could dramatically impact the transcytoplasmic signaling pathways. We compared calcium release and influx responses at the population and single cell level in normal and steatotic model mast cells. At the population level, all aspects of FcÃ??RI-dependent calcium mobilization, as well as activation of calcium-dependent downstream signaling targets such as NFATC1 phosphorylation are suppressed. At the single cell level, we demonstrate that LB are both sources and sinks of calcium following FcÃ??RI cross-linking. Unbiased analysis of the impact of the presence of LB on the rate of trans-cytoplasmic calcium signals suggest that LB enrichment accelerates calcium propagation, which may reflect a Bernoulli effect. LB abundance thus impacts this fundamental signaling pathway and its downstream targets.
|
| URL Link : |
http://www.ncbi.nlm.nih.gov/pubmed/25016314
|
| PMID : |
25016314
|
| PMCID : |
PMC4191928
|
|
|
Supported by a grant from the National Institute on Minority Health and Health Disparities (U54MD007584), National Institutes of Health.
